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  • Title: Preparation of radioactive iodinated cholylhistamine for use in the radioimmunoassay of cholic acid.
    Author: Weinberg PB, Kinkade JM, Collins DC.
    Journal: Steroids; 1977 Nov; 30(5):637-45. PubMed ID: 611631.
    Abstract:
    A major handicap in the development of simple and accurate radioimmunoassay procedures for bile acids has been the lack of a radioactive standard of high specific activity. To provide such a compound, we first synthesized cholylhistamine using the carbodiimide reaction. The hypothesized structure was confirmed by elemental analysis, thin-layer chromatography, infrared and mass spectral analysis. The cholylhistamine was then iodinated with 125I, using the choloramine-T method. The 125I-cholylhistamine was bound by antisera raised against a cholic acid-bovine serum albumin conjugate. This procedure should prove useful in preparing radioactive conjugates for all of the bile acids.
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