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  • Title: [Stable compound of inorganic pyrophosphatase with pyrophosphate obtained by a fluoride-mediated reaction with phosphate].
    Author: Bakuleva NP, Baikov AA, Avaeva SM.
    Journal: Biokhimiia; 1981 Sep; 46(9):1674-80. PubMed ID: 6117333.
    Abstract:
    Incubation of inorganic pyrophosphate from baker's yeast with phosphate and MgCl2 in the presence of fluoride results in a gradual inactivation of the enzyme concomitant with incorporation of PP1 (about 2 moles per mole) into the protein. The rate constant for this process shows an increase with a rise in concentrations of the three reagents, the maximal value of inactivation being 0.11 min-1. The bound PP1 is not separated by gel-filtration. The rate of spontaneous degradation of the enzyme-pyrophosphate complex and the nature of EDTA and Mg2+ effects are similar to those for the analogous compound obtained by inhibition of PP1 hydrolysis by fluoride. The data obtained suggest that during PP1 synthesis and hydrolysis by pyrophosphatase fluoride stabilizes the same intermediate of the enzyme with pyrophosphate.
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