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  • Title: A radioimmunoassay for bovine parathyroid hormone.
    Author: Garel JM, Barlet JP.
    Journal: J Physiol (Paris); 1976; 72(2):249-57. PubMed ID: 61269.
    Abstract:
    A radioimmunoassay for bovine parathyroid hormone (bPTH) has been developed. An antibody was raised in a goat against 1-84 b PTH which was directed against the carboxy-terminal part of the molecule (no cross-reactivity with synthetic 1-34 b PTH fragment). 1-84 b PTH was labelled with 125I using the chloramine-T method. The tubes were incubated at 4 degrees C for 6 days in an equilibrium system with 25% protein concentration. Separation was performed using plasma-coated charcoal. Jugular venous plasma PTH levels were shown to be increased in hypocalcemic parturient cows.
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