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  • Title: The nicotinic acetylcholine receptor from Discopyge tschudii: purification, characterization and reconstitution into liposomes.
    Author: Ochoa EL, de Jiménez Bonino MB, Cascone O, Medrano S, Cousseau MB.
    Journal: Comp Biochem Physiol C Comp Pharmacol Toxicol; 1983; 76(2):313-7. PubMed ID: 6140114.
    Abstract:
    The nicotinic acetylcholine receptor from Discopyge tschudii electroplax was purified by affinity chromatography on Affi-Gel 401 using bromoacetylcholine as the ligand. Its specific activity was about 4000 pmol 125I-alpha-bungarotoxin/mg protein. SDS-polyacrylamide gel electrophoresis revealed four bands of apparent molecular weights: 41,200, 49,500, 60,000 and 66,300. The amino acid composition of each individual subunit was determined. Native membranes, rich in nicotinic receptor exhibited carbamylcholine-catalysed cation transport (blocked by curare and desensitized by prior incubation with the cholinergic agonist). The functional activity of the purified material could be reconstituted into soybean lecithin liposomes. Our data show that the Discopyge tschudii nicotinic receptor is similar to that from Torpedo californica.
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