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  • Title: The assay of tropane derivatives in formulations by second derivative ultraviolet spectrophotometry.
    Author: Hassan SM, Davidson AG.
    Journal: J Pharm Pharmacol; 1984 Jan; 36(1):7-10. PubMed ID: 6141274.
    Abstract:
    The development of a second order derivative spectrophotometric assay of atropine, hyoscine and benztropine in formulations is described. The assay involves the extraction into 1,2-dichloroethane of the tetraphenylborate salt of the alkaloids precipitated from pH 6 solution by an excess of sodium tetraphenylboron and measurement of the amplitude near 273 nm to its shorter wavelength satellite in the second derivative spectrum. The second derivative amplitude gives a more precise determination of the alkaloids than absorbance. A two-point bracketing standardization of the amplitude is required owing to a small but significant intercept in the calibration data. The procedures are sufficiently sensitive and precise for the batch and unit-dose assay of tablets of atropine sulphate (0.6 mg) hyoscine hydrobromide (0.3 mg) and benztropine mesylate (2 mg), and for the assay of atropine sulphate injection (0.4 mg ml-1) and tincture of belladonna.
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