These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Biochemistry and molecular biology of catecholamine neurons: a single gene or gene family hypothesis.
    Author: Joh TH, Baetge EE, Ross ME, Reis DJ.
    Journal: Clin Exp Hypertens A; 1984; 6(1-2):11-21. PubMed ID: 6141853.
    Abstract:
    We postulate that the catecholamine synthesizing enzyme, tyrosine hydroxylase (TH), dopamine B-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) are coded for by similar gene coding sequence(s). This is based on the following observations: (1) proteolytic digestion of these enzymes produces similar peptides whose amino acid composition is nearly identical; (2) antibodies to each enzyme coprecipitate more than one of these enzymes from in vitro poly(A)mRNA translation products; (3) using hybrid selection analysis to positively identify the cDNA clones, we discovered that DBH cDNA clones cross-hybridize with PNMT mRNA, and PNMT cDNA cross-hybridize with DBH mRNA; (4) DNA hybridization analysis demonstrated that DBH and PNMT cDNA probes hybridized to several common restriction fragments of total cellular DNA. The evidence suggests that these enzymes are coded for by a single gene or linked genes coding for all catecholamine enzymes.
    [Abstract] [Full Text] [Related] [New Search]