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  • Title: A high-performance liquid chromatography assay for asparagine synthetase.
    Author: Unnithan S, Moraga DA, Schuster SM.
    Journal: Anal Biochem; 1984 Jan; 136(1):195-201. PubMed ID: 6143517.
    Abstract:
    A highly sensitive method for assaying asparagine synthetase and its glutaminase activity is presented. The amino acids L-asparagine, L-aspartate, L-glutamate, and L-glutamine, are separated by derivatization with o-phthaldialdehyde followed by reversed-phase high-performance liquid chromatography on an Altex ultrasphere-ODS C18 column. The elution is isocratic and the mobile phase used is 50 mM sodium acetate buffer (pH 5.9) with 30% methanol. This assay can easily detect picomoles of asparagine, which may be difficult to do with the other assays that have been described.
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