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  • Title: Influence of myosin antiserum on heart sarcolemmal Ca2+ or Mg2+ ATPase activity.
    Author: Tuana BS, Kutryk MJ, Elimban V, Wikman-Coffelt J, Dhalla NS.
    Journal: Res Commun Chem Pathol Pharmacol; 1984 Aug; 45(2):297-300. PubMed ID: 6148777.
    Abstract:
    Sarcolemmal Ca2+/Mg2+ ATPase was inhibited only about 30% by myosin antiserum that decreased myofibrillar ATPase activity by about 80%. There was a remarkable difference in the effect of myosin antiserum on sarcolemmal Ca2+/Mg2+ ATPase and myofibrillar ATPase with regard to its pre-incubation time with these organelles. Tryptic digestion of the sarcolemmal membrane did not show any change in its inhibitory effect of the myosin antiserum on Ca2+ ATPase. The data distinguish the Ca2+/Mg2+ ATPase from myosin ATPase and suggest that it is an enzyme of the sarcolemmal origin.
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