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  • Title: Technical notes on acrylamide gel electrophoresis used for comparing isozymes of mosquito larvae.
    Author: Tsukamoto M.
    Journal: J UOEH; 1984 Jun 01; 6(2):193-204. PubMed ID: 6148783.
    Abstract:
    Various slight modifications of electrophoretic techniques were tried to obtain more reproducible results for comparing several isozymes with individual mosquito larvae. Horizontal electrophoresis was done using polyacrylamide gel plates of 1 mm thickness on a simple cooling system in which ice-cold water was circulated. This made it possible to carry out electrophoresis effectively even during field work in tropical areas. Gel concentration in routine work is 5% for usual enzymes, but for esterase, 6% gels have been adopted. Gel concentrations, running distance, running time, visualization of isozyme bands, etc., have especially been considered.
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