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  • Title: Effect of 2-deoxy-D-glucose on maintenance in culture of neonatal B cell of rat.
    Author: Yoshida K, Kagawa S, Murakoso K, Matsuoka A.
    Journal: In Vitro; 1984 Oct; 20(10):756-62. PubMed ID: 6151542.
    Abstract:
    The effect of 2-deoxy-D-glucose on maintenance in culture of B cells of the neonatal rat was examined by supplementation of Medium 199 containing 5.5 mM glucose with 1 mM 2-deoxy-D-glucose. Islets maintained in medium with 5.5 mM glucose (basal medium) for 7 d underwent remarkable decreases in glucose sensitivity, and the levels of insulin in the medium dropped. By contrast, addition of 2-deoxy-D-glucose promoted a higher insulin content in medium and an increase in the glucose-induced insulin release and biosynthesis. Moreover, the addition of the deoxysugar caused a selective deletion of fibroblasts and prevented the deterioration of islet cells in basal medium, yielding clusters mostly consisting of islet cells at the end of culture.
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