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  • Title: In vitro protein synthesis activity of poly(A) RNA from neuroblastoma cells.
    Author: Cuppelo A, Dierich A, Mandel P, Wintzerith M.
    Journal: Neurochem Res; 1980 Mar; 5(3):271-9. PubMed ID: 6154901.
    Abstract:
    Protein synthesis activity of poly(A) RNA from M1 neuroblastoma cells was studied in a reticulocyte cell-free system. The activity of poly(A) RNA from M1 cells "differentiated" morphologically by bromodeoxyuridine was compared with that of proliferating cells. Poly(A) RNA from differentiated cells was about 10% more active than the corresponding RNA from proliferating cells. The products synthesized in vitro were fractionated by polyacrylamide gradient gel electrophoresis. A 420,000-dalton band was present in the translation products programmed by differentiated cell poly(A) RNA; it was absent in the translation products programmed by proliferating cell poly(A) RNA.
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