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  • Title: [Cultivation of herpesvirus in suspension culture on a microcarrier].
    Author: Bektemirov TA, Nagieva FG.
    Journal: Vopr Virusol; 1980; (5):615-8. PubMed ID: 6159739.
    Abstract:
    The time course of RK-13 cell growth on a microcarrier DEAE-sephadex A-50 treated by different methods was studied. Treatment of the microcarrier according to the modified method was shown to provide the best conditions for cell attachment and growth. Herpes virus type I in monolayer cultures reached maximum titers at 2--3 days depending on the multiplicity of infection (6.5--7.83 lg CPD50). In cell cultures on the microcarrier at a high multiplicity of infection the virus titer reached maximum values (9.5 lg CPD50) as early as 24 hours postinfection.
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