These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins.
    Author: Pileri S, Serra L, Martinelli G.
    Journal: Basic Appl Histochem; 1980; 24(3):203-7. PubMed ID: 6160845.
    Abstract:
    Application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlabelled antibody peroxidase-antiperoxidase (PAP) method. This study shows that treatment of tissue sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior to performance of the PAP procedure greatly enhances its sensitivity. The optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given.
    [Abstract] [Full Text] [Related] [New Search]