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  • Title: Hydrolysis of galactose from glycolipids and glycoprotein by young rat brain beta-galactosidases immobilized to Sepharose 4B.
    Author: Yeung KK, McKinney RA, Dain JA.
    Journal: J Neurochem; 1980 Aug; 35(2):407-11. PubMed ID: 6161222.
    Abstract:
    An extract of glycosidic enzymes from young rat brain was immobilized to cyanogen bromide-activated Sepharose 4B. Most glycosidases retained approximately 10--25% of their activities after immobilization. Immobilized beta-galactosidases were used repeatedly without detectable loss of enzyme activity in the hydrolysis of p-nitrophenyl-beta-D-galactopyranoside. In addition to the synthetic substrate, the immobilized rat brain beta-galactosidases could also hydrolyze galactose from lactose, galactosylcerebroside, asialofetuin, and GM1-ganglioside. The hydrolysis of GM1- to GM2-ganglioside was confirmed on TLC.
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