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  • Title: Human fibroblast interferon. An improved purification.
    Author: Knight E, Fahey D.
    Journal: J Biol Chem; 1981 Apr 25; 256(8):3609-11. PubMed ID: 6163770.
    Abstract:
    Human fibroblast interferon has been purified 2,900-fold to homogeneity. The purification is achieved in two steps by chromatography on blue Sepharose. The specific activity of the homogeneous interferon is 5 X 10(8) units/mg and the yield of biological activity has ranged from 20-40%. The interferon can exist as a monomer (Mr = 20,000) and as a dimer (Mr - 40,000). The dimer can be converted to the monomer by heating in sodium dodecyl sulfate and thioglycolic acid.
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