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  • Title: Vasoactive intestinal polypeptide-like immunoreactivity in a human neuroblastoma cell line and the coexistence of other neuropeptide immunoreactivity in the cell line.
    Author: Yanaihara N, Kobayashi S, Sato H, Yanaihara C, Sakagami M, Sakura N, Hashimoto T, Ishikawa S, Iwanaga T, Fujita T.
    Journal: Endocrinol Jpn; 1980 Dec; 27 Suppl 1():37-42. PubMed ID: 6164547.
    Abstract:
    The presence of VIP-like immunoreactivity in human neuroblastoma cell line NB-1 was demonstrated by radioimmunoassay of the crude cell extract and by immunocytochemical staining of the cells. Gel filtration profiles of VIP-like immunoreactivity in the cell extract measured by radioimmunoassays using three different region-specific antisera revealed that the immunoreactivity consists of a major molecular form corresponding to porcine VIP having 28 amino acid residues with at least two additional minor forms larger and smaller than the VIP. In addition to VIP-like immunoreactivity, the cell extract was shown to contain substance P-, neurotensin- and somatostatin-like immunoreactivity as well.
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