These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Control on the absence of DEAE-polysaccharides in DEAE-sephadex purified poliovirus suspensions by pyrolysis mass spectrometry.
    Author: Windig W, Haverkamp J, van Wezel AL.
    Journal: Dev Biol Stand; 1981; 47():169-77. PubMed ID: 6164585.
    Abstract:
    Poliovirus suspensions produced in microcarrier culture of primary monkey kidney cells and purified by column chromatography on DEAE-sephadex have been tested for the presence of DEAE-polysaccharides (in essence DEAE-dextran) by pyrolysis mass spectrometry. Semi-quantitative evaluation of the pyrolysis mass spectra indicates that the concentration of DEAE-dextran in the purified poliovirus concentrates is below 20 ppm i.e. the lower detection level of the method. As the final vaccines contain a 1:50 to 1:100 dilution of the tested virus suspension, the DEAE-polysaccharide concentration in the vaccine will be below 1 ppm at least. Pyrolysis mass spectrometry appeared to be a vary suitable method for control on the absence of this component.
    [Abstract] [Full Text] [Related] [New Search]