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  • Title: Purification and characterization of kallikrein from plasma of patients with acute pancreatitis.
    Author: Toki N, Sumi H, Takasugi S.
    Journal: Clin Sci (Lond); 1981 Feb; 60(2):199-205. PubMed ID: 6165511.
    Abstract:
    1. A kallikrein-like enzyme in plasma of patients with acute pancreatitis was further purified by successive hydroxyapatite/cellulose and Sepharose-4B column chromatography. 2. By these procedures 0.26 mg of purified enzyme with a specific activity of 215 S-2266 chromozyme units/mg of protein was obtained from 10 ml of original plasma. 3. The purified material was homogeneous as ascertained by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and had an apparent molecular weight of 31 000 as measured by gel filtration on Sephadex G-200. 4. It was confirmed immunologically that this enzyme was pancreatic kallikrein, which is distinct from plasma kallikrein, and that it could combine with alpha 2-macroglobulin only in the presence of trypsin.
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