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  • Title: [Relationship between catalytic properties, fixed charge concentration and electrostatic potential of polyelectrolyte-bound enzymes].
    Author: Fischer J, Schellenberger A, Lasch J.
    Journal: Acta Biol Med Ger; 1980; 39(11-12):1111-9. PubMed ID: 6166140.
    Abstract:
    An equation for the calculation of the electrostatic potentials of polyelectrolyte-enzyme supports from electrostatic parameters has been derived by relating two different theories which describe the catalytic behaviour of polyelectrolyte-bound enzymes. The electrostatic potentials of polyionic supports have been determined by use of experimental results, on the one hand, from the fixed charge concentration and the ionic strength, on the other hand, from pH- and Km-shifts of immobilized enzymes. The accordance of potentials calculated from electrostatic and kinetic parameters confirms the macroscopic carrier-enzyme model.
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