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  • Title: [Mechanism of AT base pairs recognition by molecules of dye "Hoechst 33258"].
    Author: Mikhaĭlov MV, Zasedatelev AS, Krylov AS, Gurskiĭ GV.
    Journal: Mol Biol (Mosk); 1981; 15(3):690-705. PubMed ID: 6166855.
    Abstract:
    Experimental studies are reported on the binding of the dye "Hoechst 33 258" to DNA and synthetic polynucleotides. A stereochemical model of binding of the dye to DNA is proposed. According to the model, the molecules of the dye are located in the minor groove of DNA, each covering 4 base pairs. The benzimidazole backbone of the dye tends to form a helix isogeometric to that of DNA in the B form. The specifisity of "Hoechst 33 258" binding to At pairs involves hydrogen bonding of two NH-groups of benzimidazole rings and CH3N+H group of the piperazine ring to O2 oxygenes of thymines and N3 nitrogens of adenines facing the minor groove of the DNA double helix.
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