These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A polyacrylamide gel method for the cytochemical demonstration of glucose-6-phosphate dehydrogenase activity in mouse sperm.
    Author: Zimmermann N, Geyer G.
    Journal: Acta Histochem; 1981; 68(2):227-30. PubMed ID: 6168161.
    Abstract:
    Solubility and low activity of glucose-6-phosphate dehydrogenase let both the aqueous and agarose gel cytochemical incubation techniques fail in mouse sperm. By means of a polyacrylamide gel medium G6PDH was constantly demonstrated in the midpieces of sperm cells prefixed with cold acetone. Although this technique prevented the dislocation of the enzyme, diffusion artifacts due to delayed formation of formazan may occur from sites of high dehydrogenase activity, e.g., epididymal epithelium encountered in sperm smears.
    [Abstract] [Full Text] [Related] [New Search]