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  • Title: Study of salivary gland specific transcripts in Chironomus tentans by cDNA analysis.
    Author: Büchner-Vollmers A, Hollenberg CP.
    Journal: Chromosoma; 1981; 83(3):315-25. PubMed ID: 6168443.
    Abstract:
    Preparative amounts of polyA-containing RNA were purified from isolated salivary glands from Chironomus tentans and used as a template for the synthesis of cDNA by AMV reverse transcriptase. The yield of cDNA corresponded to 8-14% of the input polyA RNA. Using the Alwine hybridization method (Alwine et al., 1977) it was shown that only a minor part of the cDNA hybridized to the 75S RNA band and that the major hybridization occurred to a hitherto unknown gland-specific polyA RNA transcript of 1.3 kb length. In addition, the cDNA hybridized to a gland-specific polyA-RNA transcript of 2 kb length. - The majority of the cDNA had a size of 0.4 or 0.7 kb in equal amounts. A Southern hybridization of the cDNA to an EcoRI digest of genomic DNA showed in addition to a very weak hybridization to the 40 kb band of the Balbiani ring genes, hybridization to several restriction fragments with sizes from 1.3 to 16 kb.
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