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  • Title: Axoplasmic transport of microtubule-associated proteins in the rat sciatic nerve.
    Author: Takenaka T, Inomata K.
    Journal: J Neurobiol; 1981 Sep; 12(5):479-86. PubMed ID: 6168742.
    Abstract:
    32P-ATP was injected into the L5 dorsal root ganglion and axoplasmic transport of the phosphorylate MA proteins 2, microtubule-associated proteins 2, was observed. After the injection of 32P-ATP, the nerve was dissected out at prescribed time intervals and sliced into 5-mm pieces. Each segment was electrophoresed on an SDS-polyacrylamide slab gel and subjected to autoradiography. A protein of 310,000 dalton was transported at a velocity of 6.6-10.6 mm/day in the axon with the electrophoretic mobility identical to that of MA proteins 2, one of the key components associated with the microtubules.
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