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  • Title: Assay of defective-interfering semliki forest virus by the inhibition of synthesis of virus-specified RNAs.
    Author: Barrett AD, Crouch CF, Dimmock NJ.
    Journal: J Gen Virol; 1981 Jun; 54(Pt 2):273-80. PubMed ID: 6169791.
    Abstract:
    We describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.
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