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  • Title: Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine.
    Author: Beck HP.
    Journal: Cytometry; 1981 Nov; 2(3):170-4. PubMed ID: 6170499.
    Abstract:
    A simple analytical method is described for the evaluation of flow cytometric data of perturbed cell populations obtained by applying the BrdUrd-33258 Hoechst technique. This procedure allows for determining the growth curve, the efflux from and the influx into G2 + M as well as the transit characteristics of cell cohorts through the G2 + M phase. As an example of the application of the method, the radiotoxic effects of incorporated 3H-thymidine on the proliferation of L-929 cells in vitro are described.
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