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  • Title: Effect of alpha-glucosidase inhibitor on human pancreatic and salivary alpha-amylase.
    Author: Suehiro I, Otsuki M, Yamasaki T, Ohki A, Sakamoto C, Yuu H, Maeda M, Baba S.
    Journal: Clin Chim Acta; 1981 Dec 09; 117(2):145-52. PubMed ID: 6171367.
    Abstract:
    The mode of inhibition of a new complex oligosaccharide that inhibits the alpha-glucoside hydrolase activity of pancreatic and salivary alpha-amylase was studied. Kinetic analysis revealed a non-competitive type of inhibition with a Ki of 1.47 +/- 0.03 micrograms when tested against human pancreatic alpha-amylase and 3.89 +/- 0.08 micrograms against human salivary alpha-amylase. The inhibitory action of alpha-glucoside hydrolase inhibitor (alpha-GHI) on pancreatic amylase was observed over a wide range of pH (6.0--7.9), whereas the inhibition of salivary amylase was optimal at pH 6.5. Column chromatographic investigations suggested the possible formation of an enzyme-inhibitor complex because the mixture of alpha-GHI and pancreatic alpha-amylase was eluted as a single component through a Sephadex G200 column. However, this enzyme-inhibitor complex was easily separated into each component and the enzyme activity was fully recovered after electrophoresis.
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