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  • Title: Delayed-type hypersensitivity in rabbits. Comparison of the adjuvants dimethyl dioctadecyl ammonium bromide and Freund's complete adjuvant.
    Author: Snippe H, de Reuver MJ, Beunder JW, van der Meer JB, van Wichen DF, Willers JM.
    Journal: Int Arch Allergy Appl Immunol; 1982; 67(2):139-44. PubMed ID: 6173338.
    Abstract:
    Injection of rabbits with antigen mixed with the cationic surface-active lipid dimethyl dioctadecyl ammonium bromide (DDA) induced delayed-type hypersensitivity (DH), which could be measured as skin reactions and was confirmed by histology of the skin test sites. 1 week after injection of a conjugate of bovine serum albumine (BSA) with dinitrophenol (DNP30-BSAj) mixed with DDA, DH was detectable in most but not in all rabbits. Similar results were obtained using FCA as adjuvant. The animals treated with the latter adjuvant, however, produced a long-lasting DH (1-3 weeks) complicated by circulating anti-DNP antibodies (Arthus-type reactions). Skin testing with heterologous hapten-carrier complexes revealed that individual rabbits immunized with DNP30-BSA in DDA expressed DH with different reaction patterns, either to hapten, carrier or both. In conclusion, DDA is a useful adjuvant for the induction of a state of pure DH in rabbits. However, not all rabbits do respond, or respond similarly.
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