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  • Title: [Relationship between morological variability of Bacillus subtilis R-623 and biosynthesis of hydrolytic enzymes].
    Author: Bakhmatova IB, Glemzha AA, Zharikova GG.
    Journal: Prikl Biokhim Mikrobiol; 1981; 17(3):415-21. PubMed ID: 6174968.
    Abstract:
    By synthetic sorbent chromatography the influence of Bacillus subtilis R-623 morphology on the qualitative and quantitative composition of alpha-amylase and proteases was studied. It was found that morphological variants of natural variability of Bac. subtilis R-623, alpha-amylase producer, differed in their cultural, morphological and physiological properties as well as in the amount of hydrolytic enzymes synthesized per unit of the cultural medium. Cells of P variant produced the highest quantity of alpha-amylase (314 U/lm) and cells of P and M variants synthesized the greatest amount of proteases (4.3 and 4.0 U/ml, respectively). Quantitative variations of alpha-amylase and proteases were measured in the cultural medium of morphological variants of Bac. subtilis R-623 during cultivation. Qualitative composition of those enzymes was determined when their content in the cultural medium was at the highest level. R variant synthesized alpha-amylase and protease, P and S variants alpha-amylase and two proteases, and P and S variants alpha-amylase and two proteases, and M variant one protease.
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