These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Multiplication-stimulating activity (MSA) and cartilage-derived factor (CDF): biological actions in cultured chondrocytes.
    Author: Kato Y, Nomura Y, Tsuji M, Ohmae H, Nakazawa T, Suzuki F.
    Journal: J Biochem; 1981 Nov; 90(5):1377-86. PubMed ID: 6175620.
    Abstract:
    Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activity (MSA), which is thought to be included in somatomedins. CDF and MSA markedly increased [3H]thymidine incorporation into rabbit costal chondrocytes from 7--14 h after their additions. The incorporation of [3H]uridine was also increased within 1--2 h in the cells incubated with the peptides. These stimulations of nucleic acid syntheses were followed by cell division in serum-free culture medium. On the other hand, the peptides enhanced the synthesis of sulfated proteoglycans within 7 h. Furthermore, the extent of stimulation of proteoglycan synthesis, as measured in terms of increased incorporation of [35S]sulfate, [3H]glucosamine, or [3H]serine into material precipitated with cetylpyridinium chloride, was consistently higher than the extent of CDF or MSA stimulation of total protein synthesis, as measured in terms of increased incorporation of [3H]serine into acid-insoluble cellular material. These findings suggest that the stimulation of proteoglycan synthesis by CDF and MSA is not directly linked to the peptide-induced stimulation of cell proliferation. The present study also showed that CDF is as effective as MSA in stimulating various metabolic indices in cultured chondrocytes at their optimal concentrations.
    [Abstract] [Full Text] [Related] [New Search]