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  • Title: Restriction map of poliovirus type 2 cDNA.
    Author: Kopecka H, van der Werf S, Bregegere F, Kitamura N, Dreano M, Rothberg PG, Wimmer E, Kourilsky P, Girard M.
    Journal: Dev Biol Stand; 1981; 50():301-9. PubMed ID: 6176488.
    Abstract:
    Poliovirus type 1 RNA was reverse-transcribed into c-DNA and inserted at the Pst I site of the plasmid vector pBR322 of E. coli. Resulting recombinant plasmids were analyzed by hybridization with RNase T1-resistant 32P-labeled oligonucleotides, and by restriction enzyme mapping. All of the genome was cloned in a series overlapping cDNA inserts, the longest of which was 3.2 kb. The restriction map of the poliovirus cDNA is presented.
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