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  • Title: Fate of digesta ribonucleic acid in small intestine of sheep.
    Author: Ben-Ghedalia D.
    Journal: J Dairy Sci; 1981 Dec; 64(12):2422-5. PubMed ID: 6176604.
    Abstract:
    A new approach for measurement of ribonucleic acid is based on extraction of ribonucleic acid by combined action of a detergent and high speed homogenization followed by precipitation of ribonucleic acid with cadmium +2 ions and its subsequent measure with orcinol. Disappearance of digesta ribonucleic acid flowing along the small intestine was measured in six rams, each equipped with three T-shaped cannulas that were placed in six sites of the small intestine. Chromic oxide was a marker to measure rate of flow of digesta. Net disappearance of ribonucleic acid was completed 7 m from the pylorus, amounting to 83.9% of the quantity which entered the duodenum. About 50% of the net degraded ribonucleic acid disappeared in the 1st m of the small intestine.
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