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  • Title: Comparison of normal and Bernard-Soulier platelet membrane glycoproteins: isoelectric characteristics and surface radiolabel.
    Author: Peterson DM, Hirst A, Wehring B.
    Journal: J Lab Clin Med; 1982 Jul; 100(1):26-36. PubMed ID: 6177810.
    Abstract:
    The protein and glycoprotein compositions of platelets from normal individuals, patients with B-S disease, and subjects heterozygous for B-S were determined by two-dimensional gel electrophoretic analysis of platelets that had been radiolabeled on the surface by lactoperoxidase-catalyzed iodination or by the periodate-borohydride reaction. In normal platelets, five PAS-stained glycoproteins were found in the GP I region of the two-dimensional gels. B-S platelets contained four of the glycoproteins of the GP I complex but were deficient in one glycoprotein (Mr = 139,000 and pI 6.36) that, in normal platelets, was not stained by Coomassie blue but had a high affinity for both PAS stain and alcian blue stain and was heavily labeled on the surface of platelets by the periodate-borohydride reaction but only faintly with radioiodine. Platelets from three heterozygous B-S individuals did not differ from normal controls by these techniques. These results further define the glycoprotein composition of normal platelets and show a specific deficiency of a portion of the GP I complex in B-S platelets.
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