These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Development and clinical evaluation of two immunoassays for prostatic acid phosphatase in serum.
    Author: de Vries GP, Sanders GT.
    Journal: Am J Clin Pathol; 1982 Aug; 78(2):189-95. PubMed ID: 6179425.
    Abstract:
    Polystyrene tubes were coated with IgG, isolated from antiserum against human prostatic acid phosphatase, and incubated with a known amount of purified antigen as a standard, and sera from patients. The amount of bound prostatic acid phosphatase was established by using its enzyme activity. This was performed either spectrophotometrically with p-nitrophenylphosphate (enzyme immunoassay) or fluorometrically with alpha-naphthylphosphate as a substrate (immunofluorescence assay). Results of both methods were compared with those of the conventional method and were evaluated in four groups of patients with: non-prostatic disease, hypertrophy, treated and untreated prostatic carcinoma. The first group was used to establish the upper limit of normal. In the hypertrophy group, the specificity of the immunological methods when compared with the conventional method, improved from 79% to 91%. Sensitivity, calculated from the untreated prostatic carcinoma group, was 74% for the enzyme immunoassay (EIA) and 71% for the immunofluorescence assay (IFA). The probability of having either carcinoma or hypertrophy, given observed EIA or IFA values, was calculated by the statistical method of the logistic regression.
    [Abstract] [Full Text] [Related] [New Search]