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  • Title: A reliable and sensitive method for the simultaneous determination of dopamine, noradrenaline, 5-hydroxytryptamine and 5-hydroxy-indolacetic acid in small brain samples.
    Author: Orsingher OA, Marichich ES, Molina VA, Ramírez OA.
    Journal: Acta Physiol Lat Am; 1980; 30(2):111-5. PubMed ID: 6180600.
    Abstract:
    A sensitive and reliable fluorometric method for the simultaneous determination of dopamine, noradrenaline, 5-hydroxytryptamine and 5-hydroxy-indol acetic acid in small samples of brain tissues is described. The procedure is based on solvent extraction; catecholamines are oxidized by the Chang's method and 5-hydroxytryptamine and 5-hydroxy-indol acetic acid determined by reaction with o-phthalaldehyde, alpha-methyl-p-tyrosine causes a negligible interference with the procedure. Results of determination of these amines in different brain areas are reported.
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