These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Lack of expression of HLA-ABC antigens in choriocarcinoma and other human tumor cell lines.
    Author: Travers PJ, Arklie JL, Trowsdale J, Patillo RA, Bodmer WF.
    Journal: Natl Cancer Inst Monogr; 1982; 60():175-80. PubMed ID: 6181407.
    Abstract:
    We studied a panel of 21 human tumor and other cell lines, mainly of epithelial origin, for the expression of HLA-ABC antigens by an indirect radiobinding assay using monoclonal antibodies. Four of the lines, 2 choriocarcinomas, a breast tumor cell lin (MDA-MB-157), and a colon carcinoma (LoVo) showed no detectable reaction with the antibodies. The levels of binding in another breast tumor line (SkBr3) and a colon carcinoma line (LS174T) were much reduced. These cell lines plus some of the HLA-ABC positive lines from the panel were studied by immunoprecipitation of 35S-labeled cell extracts. Two mechanisms for alteration of the HLA-ABC antigen levels at the cell surface were apparent. In the choriocarcinomas and one breast tumor line, beta 2 microglobulin (beta 2m) was expressed but little or no HLA-A, B, or C polypeptides were synthesized. The other HLA-ABC negative lines had defects similar to that in the previously reported Burkitt's lymphoma cell line DAUDI. In these lines, HLA-ABC polypeptides were produced but did not appear at the cell surface because beta 2m was not synthesized. The absence of HLA-ABC determinants from the cell surface is presumed to afford protection against T-cell immune attack whether naturally as against the trophoblast during pregnancy or in abnormal situations against certain tumors.
    [Abstract] [Full Text] [Related] [New Search]