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  • Title: [Changes in the representation of repeating DNA sequences in nuclear RNAs of early loach embryos].
    Author: Bronshteĭn IB, Gazarian KG, Davitashvili AN, Kafiani KA, Strelkov LA.
    Journal: Biokhimiia; 1982 Aug; 47(8):1262-71. PubMed ID: 6181818.
    Abstract:
    The hybridization kinetics of nuclear RNAs of loach embryos labelled with [3H]uridine for 1 hour with DNA excess shows that during embryogenesis (from the blastula to the gastrula stage) the number of newly formed RNA molecules transcribed from repeating DNA sequences in considerably reduced. This occurs both in the RNA fraction extracted from the nuclei with phenol pH 7.7 and having a low sedimentation coefficient and a low degree of polyadenylation, and in the RNA fraction extracted with phenol pH 9.0 having a higher sedimentation coefficient and a higher degree of polyadenylation.
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