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  • Title: Comparison of growth rates of two clones of Bacillus subtilis, producer of alpha-amylase.
    Author: Pazlarová J, Yegorova LA, Fencl Z, Loginova LG.
    Journal: Folia Microbiol (Praha); 1982; 27(5):323-7. PubMed ID: 6183179.
    Abstract:
    In a population of the productive Bacillus subtilis strain the production of alpha-amylase differentiates at the cell level. Individual cells in the population were analyzed and the production of isolates was tested. The mean specific activity of alpha-amylase in the productive group after the isolation was 5.0 U/mg dry substance and in the nonproductive group 1.5 U/mg dry substance. This ratio remained unchanged during long-term observations and after repeated transfers. In selected strains of both groups the specific growth rate was determined in a synthetic medium containing various amounts of casein hydrolyzate. The detected differences in the growth rate between the productive and the nonproductive clones are determined by amino acid concentration.
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