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  • Title: Effect of alpha-amanitin and actinomycin D on RNA synthesis in the protoplasts of Aspergillus nidulans.
    Author: Stunnenberg HG, van den Broek HW.
    Journal: Microbios; 1982; 35(141-142):197-207. PubMed ID: 6186893.
    Abstract:
    RNA synthesis was studied in protoplasts from Aspergillus nidulans, which remained metabolically active for several days under the appropriate conditions. It is concluded, that at certain actinomycin D concentrations the ribosomal RNA synthesis can be selectively inhibited. However, alpha-amanitin at concentrations up to 400 micrograms/ml neither influenced the total nor the RNA polymerase II directed RNA synthesis. It was possible to discriminate between the RNA polymerase I or II directed RNA synthesis due to the presence of poly(A) tails attached to at least part of the RNA, presumably messenger RNAs, or their precursors.
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