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  • Title: Determination of glycosylated adult and foetal haemoglobins by affinity chromatography.
    Author: Talwar D, Barr BB, Kesson CM, Robb DA.
    Journal: Clin Chim Acta; 1983 Feb 28; 128(1):61-7. PubMed ID: 6188558.
    Abstract:
    Estimation of adult glycosylated haemoglobin by affinity chromatography was found to be quick and less dependent on ionic strength, pH and temperature than ion-exchange chromatography. Results obtained by both procedures correlated strongly (r = 0.96) but the range for normal subjects was smaller with the affinity assay. The affinity method correlated equally well with the colorimetric assay (r = 0.95). However, the method did not measure all the glycosylated forms, and only half of the glycosylated species isolated by ion-exchange chromatography was bound to the affinity resin. It also showed that the amount of glycosylated haemoglobin in cord blood is less than in adult blood.
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