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  • Title: [Identification of histone mRNA and isolation of a DNA fraction enriched with Physarum polycephalum histone genes].
    Author: Bukhman VL.
    Journal: Biokhimiia; 1983 May; 48(5):768-71. PubMed ID: 6191781.
    Abstract:
    The 7-14S poly A- RNA isolated from the S-state polysomes of the macroplasmodium P. polycephalum reveals the properties of histone mRNA. In the wheat germ cell-free system this RNA is translated into the polypeptides with the same electrophoretic mobility as core histones of P. polycephalum. Using centrifugation in a CsCl density gradient with actinomycin D, a DNA fraction was isolated which hybridizes with cDNA synthesized at 7-14S poly A- RNA by reverse transcription with random priming. The DNA fraction formed can be used for cloning of P. polycephalum histone genes.
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