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  • Title: Localization of two antigenic determinants in histone H4.
    Author: Muller S, Soussanieh A, Bouley JP, Reinbolt J, Van Regenmortel MH.
    Journal: Biochim Biophys Acta; 1983 Sep 14; 747(1-2):100-6. PubMed ID: 6192844.
    Abstract:
    Four overlapping synthetic peptides corresponding to the carboxy-terminal region 80-102 of histone H4 were prepared by solid-phase peptide synthesis. Their antigenic activity was analysed by inhibition of the H4-anti-H4 reaction in complement fixation and enzyme-linked immunosorbent assay. One antigenic determinant was localized in residues 88-96 of the H4 molecule. No antigenic activity was found in peptides 80-89 and 97-102. Antibodies induced by peptide 85-102 were found to bind to free H4 in solution but not to chromatin subunits, suggesting a lack of accessibility of the C-terminal region of H4 in nucleosomes. A second epitope was found to be situated in the N-terminal region 1-53 of histone H4.
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