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  • Title: [Fractionation of a sciatic nerve homogenate from normal, trembler and quaking mice on a continuous sucrose gradient].
    Author: Darriet D, de Nechaud B, Rabaud M, Jeantet C, Cassagne C, Bourre JM.
    Journal: C R Seances Acad Sci III; 1983; 296(15):689-92. PubMed ID: 6193847.
    Abstract:
    Two fractions, characterized by biochemical criteria [quantity and density of particulate material, localization of myelin specific protein--Po, P1 and Pr--and of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) and of 5'-nucleotidase], were isolated from sciatic nerve homogenate of normal, Trembler and quaking, young and adult Mice, on a continuous sucrose gradient. The fraction, at 0.58-0.6 M, the richest in membrane particles contains the myelin. The fraction at 0.7-0.75 M should contain the plasma membrane of the Schwann cell which synthesizes myelin.
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