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  • Title: Analysis of yeast RNA polymerases with subunit-specific antibodies.
    Author: Bréant B, Huet J, Sentenac A, Fromageot P.
    Journal: J Biol Chem; 1983 Oct 10; 258(19):11968-73. PubMed ID: 6194159.
    Abstract:
    Specific antibodies directed against each polypeptide component of yeast RNA polymerases A or B were prepared and their affinity spectrum determined by protein blot immunodetection. The majority of enzyme A or B subunits were specifically recognized by their respective antiserum. A direct correspondence was established between the polypeptides immunologically related in the three forms of RNA polymerases A, B, and C by reacting the different antibodies with enzymes subunits transferred to a nitrocellulose membrane. Subunit-specific antibodies and antibodies to native enzymes A and B were used to probe the activity of RNA polymerases A, B, and C. Based on DNA protection experiments, the largest subunit of enzymes A and B as well as the common subunit ABC23 appear to be involved in DNA binding.
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