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  • Title: Purification and some properties of a plasma protein which reacts with an antiserum against urinary trypsin inhibitor-1.
    Author: Maehara S, Kawashita E, Sumi H, Toki N.
    Journal: Scand J Clin Lab Invest; 1983 Feb; 43(1):21-6. PubMed ID: 6194553.
    Abstract:
    A plasma protein which forms a precipitation line with antiserum against urinary trypsin inhibitor was purified by ammonium sulphate fractionation, anti-urinary trypsin inhibitor rabbit IgG antibody coupled sepharose immunoadsorbent column chromatography and polyacrylamide-gel disc electrophoresis. By these procedures, 0.14 mg of purified material was obtained from 20 ml of plasma. It was homogeneous as ascertained by sodium dodecyl sulphate polyacrylamide-gel disc electrophoresis and had an apparent molecular weight of 90,000. It was found that this inhibitor was immunologically distinct from inter-alpha-trypsin inhibitor.
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