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  • Title: A simple method of staining the synaptonemal complex with Coomassie brilliant blue for light microscopy.
    Author: Nanda I, Raman R.
    Journal: Stain Technol; 1983 May; 58(3):177-81. PubMed ID: 6195779.
    Abstract:
    A simple technique for staining synaptonemal complexes with Coomassie brilliant blue for light microscopy has been described. The testis cells were exposed to prolonged hypotonic treatment and dropped on Formvar-coated slides. Following fixation with paraformaldehyde the slides were stained with Coomassie brilliant blue for 15 min to 1 h at room temperature and rinsed in distilled water. For its simplicity and rapidity this technique may serve as an effective alternative to silver staining for light microscopic observation of synaptonemal complexes.
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