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  • Title: [Lyophilization of the spores and vegetative cells of Bacillus brevis var. G-B.--producer of gramicidin S].
    Author: Zarubina AP, Aslanian RR, Egorov NS.
    Journal: Antibiotiki; 1983 Aug; 28(8):605-8. PubMed ID: 6195960.
    Abstract:
    Viability, antibiotic properties and variation of 4 variants of Bac. brevis var. G.-B. were studied after lyophilization and storage for a year in the lyophilized state. It was shown that the spores and vegetative cells of S and P- variants not synthesizing gramicidin S were somewhat more stable than the spores and cells of R and P+ variants producing the antibiotic. The latter dissociated by 10 per cent towards the cells producing and not producing gramicidin. The developmental rate of the lyophilized vegetative cells was higher than that of the lyophilized spores. Under analogous cultivation conditions they produced higher amounts of the biomass and antibiotic. The lyophilization method described may be recommended for the maintenance of viability and stability of the spores and vegetative cells of Bacillus brevis var. G.-B. producing gramicidin S.
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