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  • Title: Purification and some properties of two proteases from Pseudomonas aeruginosa No. 700/75.
    Author: Krzywańska-Pajdak E.
    Journal: Acta Microbiol Pol; 1983; 32(2):131-8. PubMed ID: 6196943.
    Abstract:
    Two extracellular proteases have been isolated from the culture supernatant of a virulent strain of Pseudomonas aeruginosa. The enzymes were purified in a three-step procedure involving ammonium sulfate fractionation, acetone precipitation and column chromatography on DE-52 cellulose. The specific activity of protease I was 22.2 U/mg of protein and protease II 6.6 U/mg of protein. Immunological properties and electrophoretic mobilities of the two forms were different. The two forms differ in substrate specificity (only from I exhibited elastinolytic activity) and pH optimum (pH 7.5 and pH 10 for form I and II, respectively).
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