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  • Title: An antigen-activated DFP-inhibitable enzyme controls basophil desensitization.
    Author: Kazimierczak W, Meier HL, MacGlashan DW, Lichtenstein LM.
    Journal: J Immunol; 1984 Jan; 132(1):399-405. PubMed ID: 6197452.
    Abstract:
    Diisopropyl fluorophosphate (DFP), an inhibitor of serine esterases, enhances IgE-mediated histamine release from human basophils and blocks the desensitization process (i.e., the antigen-induced hyporesponsiveness) in these cells. Both activities occur at relatively low concentrations of DFP (0.1 to 0.5 mM) and are dependent on an antigen-activated intracellular event: if DFP is removed before antigen addition, it has neither effect. Neither hydrolyzed DFP nor the non-phosphorylating diisopropyl methyl phosphate enhanced histamine release or blocked desensitization. In addition to providing a demonstrable biochemical correlate of desensitization, our data suggest that the desensitization process controls the release of mediators of allergic reactions.
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