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  • Title: Femtogram detection limits for biogenic amines using microbore HPLC with electrochemical detection.
    Author: Caliguri EJ, Mefford IN.
    Journal: Brain Res; 1984 Mar 26; 296(1):156-9. PubMed ID: 6201234.
    Abstract:
    Detection limits for biogenic amines, serotonin (5-HT), 5-hydroxyindoleacetic acid, 5-hydroxytryptophan, norepinephrine, epinephrine, dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), of 50-200 fg are reported. This was accomplished using microbore HPLC with electrochemical detection. Reproducibility at the 1-5 pg level of 1.6-8.1% was obtained. The utility of this approach is demonstrated for analysis of brain tissues of 1 microgram or less.
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