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  • Title: Nuclear beta-globin RNA transcripts initiated in vitro with gamma-thio nucleoside triphosphate.
    Author: Weidner DA, Winicov I.
    Journal: Cell Biophys; 1983 Dec; 5(4):255-64. PubMed ID: 6202411.
    Abstract:
    We have been able to obtain significant levels of apparently in vitro-initiated beta-globin transcripts from isolated nuclei using mouse Friend cells. The level of hybrid selected beta-globin transcripts was increased 3-9-fold in nuclei after the cells were induced with dimethylsulfoxide. The newly initiated transcripts were detected through incorporation of gamma-thio ATP and isolation by Hg-agarose chromatography. The 5'-terminus was identified by recovery of the thio tetraphosphate in hydrolysis products from in vitro initiated RNA using gamma-35S ATP.
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